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KMID : 0357319920270040371
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Journal of the Korean Society for Microbiology
1992 Volume.27 No. 4 p.371 ~ p.380
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Effects of Interferon-alpha and gamma on Lymphokine-activated Killer cell Activity Induced by Interleukin-2
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Abstract
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Interleukin-2(IL-2) is a T cell derived lymphokine with a broad spectrum of growth and differentiation promoting activities. Lymphokine-activated killer(LAK) cells are generated by culture of fresh peripheral blood lymphocytes(PBL) with IL-2. LAK
cells
can lyse a wide spectrum of allogeneic and syneneic tumor cells. Recently, much attention has been paid to clinical trials of adoptive immunctherapy with LAK cells for treatment of cancer. Little is known, however, about the mechanisms regulating
LAK
cell induction by IL-2. Interferon(IFN) was discovered by virtue of its direct antiviral activity, but today IFNs are known to ifluence a wide to influence a wide variety of activities of the immune system.
To examine the effects of two classes of IFNs(IFN-α 2A nd IFN-r)on LAK activity induced by IL-2, we isolated human PBL from healthy donors and added various kinds of IFNs to cultures of lymphocytes with IL-2. Additionof excgeneous IFN-α2A to
cultures
of lymphocytes plus IL-2 inhibited LAK acitivity, but addition of IFN-r had no effect on LAK induction by IL-2. IFN-α2A also inhibited the proliferative responses of lymphocytes to IL-2 stimulation and the expression of IL-2 receptors o their
surface,
wheres IFN-r did not. Our results can provide useful information to adoptive antivancer immunotherapy.
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KEYWORD
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